产品货号：
GS4549
中文名称：
重组人Amphiregulin
英文名称：
Recombinant Human Amphiregulin
产品规格：
10μg
发货周期：
1～3天
产品价格：
询价
分子量：Approximately 11.3kDa,a single non-glycosylated polypeptide chain containing 98 amino acid residues.
储存条件：冷冻（-20℃）
概述
Amphiregulin is an EGF related growth factor and was originally isolated from the conditioned media of a PMA-treated MCF-7 human breast carcinoma cell line.It is mainly expressed numerous carcinoma cell lines and the epithelial cells of various human tissues including colon,stomach,breast,ovary,kidney,etc.Synthesized as a transmembrane protein,Amphiregulin’s extracellular domain is proteolytically processed to release the mature protein.There are 6 conserved cysteine residues,which form 3 intramolecular disulfide bonds essential for biological activity.Amphiregulin signals through the EGF/TGF-a receptor,and stimulates growth of keratinocytes,epithelial cells and some fibroblasts.It also inhibits the growth of certain carcinoma cell lines.Mutations in this encoded protein are associated with a psoriasis-like skin phenotype.
使用说明
This material is offered by Biorab Biotech for research,laboratory or further evaluation purposes.NOT FOR HUMAN USE.
技术规格
相关搜索：重组人Amphiregulin，Amphiregulin, Human，Recombinant Human Amphiregulin
储存条件：冷冻（-20℃）
概述
Amphiregulin is an EGF related growth factor and was originally isolated from the conditioned media of a PMA-treated MCF-7 human breast carcinoma cell line.It is mainly expressed numerous carcinoma cell lines and the epithelial cells of various human tissues including colon,stomach,breast,ovary,kidney,etc.Synthesized as a transmembrane protein,Amphiregulin’s extracellular domain is proteolytically processed to release the mature protein.There are 6 conserved cysteine residues,which form 3 intramolecular disulfide bonds essential for biological activity.Amphiregulin signals through the EGF/TGF-a receptor,and stimulates growth of keratinocytes,epithelial cells and some fibroblasts.It also inhibits the growth of certain carcinoma cell lines.Mutations in this encoded protein are associated with a psoriasis-like skin phenotype.
使用说明
This material is offered by Biorab Biotech for research,laboratory or further evaluation purposes.NOT FOR HUMAN USE.
技术规格
| 外观 | Sterile Filtered White lyophilized(freeze-dried)powder. |
| 纯度 | ＞95%by SDS-PAGE and HPLC analyses. |
| 来源 | Escherichia coli. |
| 生物活性 | Fully biologically active when compared to standard.The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is between 5-10ng/ml. |
| 成分 | Lyophilized from a 0.2μmfiltered concentrated solution in PBS,pH 7.4. |
| 内毒素 | Less than 1 EU/μg of rHuAmphiregulin as determined by LAL method. |
| 复溶 | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom.Reconstitute in sterile distilled water or aqueous buffer containing 0.1%BSA to a concentration of 0.1-1.0mg/mL.Stock solutions should be apportioned into working aliquots and stored at ≤ -20 ℃.Further dilutions should be made in appropriate buffered solutions. |
| 氨基酸序列 | SVRVEQVVKP PQNKTESENT SDKPKRKKKG GKNGKNRRNR KKKNPCNAEF QNFCIHGECK YIEHLEAVTC KCQQEYFGER CGEKSMKTHS MIDSSLSK |
相关搜索：重组人Amphiregulin，Amphiregulin, Human，Recombinant Human Amphiregulin